All target receptors/enzymes in this study were tested by Fish Enzyme Linked Immunosorbent Assay Kit made against Salmo salar (Solarbio Life Science, Beyotime Biotechnology and Hengyuan Biotechnology). We measured 5-hydroxytryptamine receptor 3A-like (5HT3A), acetylcholine receptor (AchR), acetylcholinesterase (AchE), acetylcholine transferase (ChAT), sodium- and chloride-dependent GABA transporter 1-like (GAT1), aromatic-L-amino-acid decarboxylase (DDC), D (5)-like dopamine receptor (DRD5), beta-2 adrenergic receptor-like (ADRβ2), sodium-dependent noradrenaline transporterlike (SLC6A2), sodium/glucose cotransporter 1-like(SGLT1),phenylethanolamine N-methyltransferase (PNMT), dopamine betamonooxygenase (DBMO), tyrosinase (TYR), and proto-oncogene (cfos). All tests were performed according to kit instructions with three biological replicates. Because antibodies used in the kits were not designed for our fish, the golden line barbel, it may underestimate the concentration due to low cross reactivity. Potassium and sodium concentration were measured with atomic absorption spectroscopy.
